Smoking cigarettes or any other tobacco nicotine source (e.g., pipe, cigar, snuff) significantly impedes the therapeutic (healing) effectiveness of Prolotherapy. 

During the course of Prolotherapy treatment sessions, it would be helpful to take supplemental Vitamin C to take advantage of its facilitating the laying down of new collagen and healing the sprain injury “wound”.

For the relief of established joint pain or post-therapeutic pain, DO NOT use any steroidal or nonsteroidal anti-inflammatory drugs (see list below)–neither prescribed, over-the-counter, oral, or injection–for two weeks prior to prolotherapy treatment and

In the case of Prolotherapy, normal activity movement, stretching, and exercise are important for the laying down of normal collagen in the healing of ligaments or tendons.

Prolotherapy is a treatment for stabilizing joint instability due to ligament sprain injury or muscle tendon sprain injury.